Fig. 3. Anti-Notch4 neutralizing mAb inhibit allergic airway inflammation in Il4RαR576 mice.
(A) scheme of antibody therapy (10μg) and airway sensitization and challenge. (B) airway hyperresponsivness in the respective mouse groups in response to methacholine. (C) serum OVA-specific IgE concentrations. (D - F) absolute numbers of lung CD4+ T cells, eosinophils and neutrophils. (G) IL-13 and IL-17 expression in lung Treg cells. (H) IL-13 and IL-17 expression in lung Teff cells. Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis (B-G). **P<0.01, ***P<0.001, ****P<0.0001.