Figure 8. Short α-syn fibrils induce inclusions in Sprague Dawley rats.
Adult (~P90) rats were unilaterally injected with 20 μg of the indicated rodent α-syn fibril preparation, or 20 μg of monomer α-syn. Six months later, rats were sacrificed and coronal sections were analyzed by immunohistochemistry for pS129-α-syn (DAB, brown coloration). A1–4) Representative images in sections adjacent to the needle track of the fibril injection. Scale bars are 200 μm. B1–4) Representative images in the ipsilateral somatosensory cortex (scale bars 100 μm), C1–4) SNpc (highlighted by dashed lines, scale bar is 200 μm), or D1–D4) amygdala (scale bar is 200 μm). Images from each brain nuclei are representative of at least three rats analyzed per group.