Figure 4. KCNQ1 and KCNE3 expression levels throughout the oestrous cycle.
A, KCNQ1 expression levels in female rat distal colonic crypts were measured by Western blot. The figure shows a representative blot of KCNQ1 proteins levels at different stages of the oestrous cycle. β-Actin expression was used as a protein loading control. The graph represents densitometric analysis of four individual experiments. B, KCNE3 expression levels in female rat distal colonic crypts were measured by Western blot. The figure shows a representative blot of KCNE3 proteins levels at different stages of the oestrous cycle. β-Actin expression was used as a protein loading control. The graph represents densitometric analysis of three individual experiments. Values are given as arbitrary units and expressed as means ± SEM. **P < 0.01, ***P < 0.001 determined by ANOVA and Tukey's post hoc test. Pro, prooestrus; Est, oestrus; Meta, metaoestrus; Dies, dioestrus.