Figure 1. Sex differences in KCNQ1 and KCNE subunits transcript expression.
Semi-quantitative RT-PCR analysis of KCNQ1 and KCNE subunit expression in male and female rat colonic crypts. Reverse transcribed cDNA from male and female colonic crypt cells was amplified using specific primers for rat KCNQ1 (A), rat KCNE1 (B), KCNE2 (C) and KCNE3 (D). The PCR reaction produced bands at the expected length for KCNQ1 and KCNE3 subunits. GADPH was used as an internal control to estimate cDNA loading. Values in graphs represent means ± SEM. *P < 001, n = 5 animals.