Fig. 2.

A dose-response study of ATRA on KLF5 mRNA levels in IEC6 cells. Northern blot analyses were conducted on RNA isolated from IEC6 cells treated with ATRA. Panel A is the result of a dose-response study in IEC6 cells treated for 3 days with ATRA with a concentration between 0 and 50 μM. Panel C is a similar experiment except that the concentration of ATRA was between 0 and 1000 nM. Blots were probed with KLF5 and then GAPDH. Panels B and D represent densitometric quantification of band intensities of KLF5 mRNA after normalizing to that of GAPDH.