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. 1979 Dec;38(3):598–608.

Relations between Fc receptor function and locomotion in human lymphocytes.

J M Shields, P C Wilkinson
PMCID: PMC1537911  PMID: 119597

Abstract

The relationship between the surface binding sites on human lymphocytes for chemotactic factors and for the Fc fraction of IgG was investigated using both blood lymphocytes and established cultures of human lymphoblasts. Pretreatment of human blood lymphocytes with a variety of chemotactic factors inhibited Fc-rosette formation. This was true even of small formylated peptides, for example, formyl-methionyl-phenylalanine (chemotactic) inhibited Fc-rosetting but unformylated methionyl-phenylalanine (non-chemotactic) did not. Conversely pretreatment of lymphocytes with IgG inhibited their locomotor reactions to a variety of chemoattractants. Aggregated IgG was more inhibitory than non-aggregated IgG and the inhibition was mediated via the Fc piece. In a filter assay, native IgG was chemokinetic but not chemotactic for lymphocytes. Heat-aggregated IgG induced more locomotion of lymphocytes than native IgG, and was possibly chemotactic, but no unequivocally so. The possibility that chemotactic factors and the Fc portion of IgG compete for the same cell surface receptor was investigated by binding studies using cultured lymphoblasts. These studies suggested that the reciprocal inhibition could not be explained by competition for receptors. An alternative explanation was suggested by the finding that inhibition of locomotion by aggregated IgG was dependent on the presence of divalent cations at the time the IgG was added, and did not occur in the presence of the calcium ionophore A23187. Addition of aggregated IgG or chemotactic factors to lymphocytes thus may lead to a gated entry of calcium, and following closure of the calcium gate, the cells become relatively unresponsive to further stimulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Foreman J. C., Garland L. G. Desensitization in the process of histamine secretion induced by antigen and dextran. J Physiol. 1974 Jun;239(2):381–391. doi: 10.1113/jphysiol.1974.sp010574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Foreman J. C., Mongar J. L. The interaction of calcium and strontium with phosphatidyl serine in the anaphylactic secretion of histamine. J Physiol. 1973 Apr;230(2):493–507. doi: 10.1113/jphysiol.1973.sp010200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fujioka S., Gallo R. C. Aminoacyl transfer RNA profiles in human myeloma cells. Blood. 1971 Aug;38(2):246–252. [PubMed] [Google Scholar]
  4. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  5. Kay N. E., Bumol T. F., Douglas S. D. Effect of phagocytosis and Fc receptor occupancy on complement-dependent neutrophil chemotaxis. J Lab Clin Med. 1978 May;91(5):850–856. [PubMed] [Google Scholar]
  6. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Scottramsey W., Zigmond S. H. A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clin Exp Immunol. 1977 Mar;27(3):377–380. [PMC free article] [PubMed] [Google Scholar]
  7. Leslie R. G., Cohen S. Cytophilic activity of IgG2 from sera of unimmunized guinea-pigs. Immunology. 1974 Oct;27(4):577–587. [PMC free article] [PubMed] [Google Scholar]
  8. Russell R. J., Wilkinson P. C., Sless F., Parrott D. M. Chemotaxis of lymphoblasts. Nature. 1975 Aug 21;256(5519):646–648. doi: 10.1038/256646a0. [DOI] [PubMed] [Google Scholar]
  9. Sandilands G. P., Gray K., Cooney A., Browning J. D. Formation of auto-rosettes by peripheral blood lymphocytes. Clin Exp Immunol. 1975 Dec;22(3):493–501. [PMC free article] [PubMed] [Google Scholar]
  10. Van Epps D. E., Williams R. C., Jr Suppression of leukocyte chemotaxis by human IgA myeloma components. J Exp Med. 1976 Nov 2;144(5):1227–1242. doi: 10.1084/jem.144.5.1227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ward P. A., Unanue E. R., Goralnick S. J., Schreiner G. F. Chemotaxis of rat lymphocytes. J Immunol. 1977 Aug;119(2):416–421. [PubMed] [Google Scholar]
  12. Wilkinson P. C. Action of sphingomyelinase C and other lipid-specific agents as inhibitors of Fc binding and locomotion in human leucocytes. Immunology. 1977 Sep;33(3):407–412. [PMC free article] [PubMed] [Google Scholar]
  13. Wilkinson P. C., Allan R. B. Binding of protein chemotactic factors to the surfaces of neutrophil leukocytes and its modification with lipid-specific bacterial toxins. Mol Cell Biochem. 1978 Jun 15;20(1):25–40. doi: 10.1007/BF00229452. [DOI] [PubMed] [Google Scholar]
  14. Wilkinson P. C., Allan R. B. Chemotaxis of neutrophil leukocytes towards substratum-bound protein attractants. Exp Cell Res. 1978 Dec;117(2):403–412. doi: 10.1016/0014-4827(78)90153-2. [DOI] [PubMed] [Google Scholar]
  15. Wilkinson P. C., Parrott D. M., Russell R. J., Sless F. Antigen-induced locomotor responses in lymphocytes. J Exp Med. 1977 May 1;145(5):1158–1168. doi: 10.1084/jem.145.5.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilkinson P. C., Roberts J. A., Russell R. J., McLoughlin M. Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes. Clin Exp Immunol. 1976 Aug;25(2):280–287. [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson P. C. Synthetic peptide chemotactic factors for neutrophils: the range of active peptides, their efficacy and inhibitory activity, and susceptibility of the cellular response to enzymes and bacterial toxins. Immunology. 1979 Mar;36(3):579–588. [PMC free article] [PubMed] [Google Scholar]
  18. Wissler J. H. Chemistry and biology of the anaphylatoxin related serum peptide system. I. Purification, crystallization and properties of classical anaphylatoxin from rat serum. Eur J Immunol. 1972 Feb;2(1):73–83. doi: 10.1002/eji.1830020115. [DOI] [PubMed] [Google Scholar]
  19. Wissler J. H., Stecher V. J., Sorkin E. Chemistry and biology of the anaphylatoxin related serum peptide system. 3. Evaluation of leucotactic activity as a property of a new peptide system with classical anaphylatoxin and cocytotaxin as components. Eur J Immunol. 1972 Feb;2(1):90–96. doi: 10.1002/eji.1830020117. [DOI] [PubMed] [Google Scholar]
  20. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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