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. Author manuscript; available in PMC: 2006 Aug 2.
Published in final edited form as: Microbes Infect. 2005 Sep 16;8(2):460–469. doi: 10.1016/j.micinf.2005.07.012

Fig. 4. Phosphorylation levels of MLC and PAK1 in HBMEC overexpressing DN-Rac1 or cAc-Rac1.

Fig. 4

Total cell lysates of pcDNA3/HBMEC (A), DN-Rac1/HBMEC (B) or cAc-Rac1/HBMEC (C) infected with OmpA+ E. coli for varying periods as indicated were analyzed by Western blot analysis using either anti-pMLC or anti-MLC antibodies. In separate experiments, the total cell lysates were immunoprecipitated with anti-phospho-PAK1 antibody and the immune complexes were analyzed by anti-phospho-PAK1 antibody. Arrows indicate either phospho-PAK1 or phospho-MLC bands. (D). The densities of phospho-PAK1 were determined from the scanned blot using ImageJ software, normalized to the densities of IgG bands, and graphed.