Figure 5.
PF-08052667 with prewash significantly enhances activity and survival benefit in IB6+ orthotopic models. Orthotopic models demonstrate consistent tumor control with PF-08052667 + prewash. A, IHC staining for IB6 in SW780 cells expressing red luciferase (left) and UM-UC-3 cells overexpressing IB6 and luciferase (right) implanted orthotopically in the bladders of female NSG and SCID mice, respectively. B, Mice bearing SW780-RL+ bladder tumors were treated with 0.1% DDM for 10 minutes immediately prior to PF-08052667 for 15 or 60 minutes (4 weekly doses) via intravesical administration. Bioluminescence signal was measured in the bladder region over time and plotted as geometric mean over time. C, AUC values are plotted to show the differences in tumor growth across treatments. Statistical analysis was performed via one-way ANOVA followed by the Tukey HSD test (Supplementary Tables S7 and S8), ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05.