Fig.4. Elicitation of BG505-WT binders through rapid immunization.
a, Experiment schematic.
b, Percent of VRC01gHL within BGC cells.
c, Total VRC01gHL BGC cells in iLNs.
d, Representative plots from each group of VRC01gHL stained with BG505-WT probe.
e, Total VRC01gHL (CD45.1+) BGC cells positive for BG505-WT probe d.
a-e, All groups contain mice from both of two experimental repeats. Final n= 8,7,9,10 left to right for groups plotted. See also Extended Data Fig.4 a-e.
f, Experiment schematic.
g, Percent VRC01gHL (CD45.1+) of total BGC cells in iLN.
h, Total VRC01gHL (CD45.1+) BGC cells positive for BG505-WT probe with brown line as Limit of Detection (LOD) at 50 cells/animal as 1/10th-1/20th of total iLNs were collected.
f-h, See also Extended Data Fig.4g-j. All groups analyzed 8 weeks post prime. Table denotes immunization weeks and contents. All immunizations included adjuvant. Immunizations match previous amounts with the exception: where (2x) or two antigens are listed final amounts of antigen and adjuvant are doubled and mice were given final immunizations at week 6 with analysis at week 7. Multiple experiments were compiled, and not all experiments included all groups. Compiled experiments include groups from Figure 5a(diamonds) and Figure 4a.
Means and SD are represented as bars. Kruskal-Wallis uncorrected test; not significant (NS), P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.