Figure 3.
Mutant KRAS drives Nesprin-3 localization to the nuclear periphery in PDAC cells. (A) Representative immunofluorescence line-scan analysis of Emerin and Nesprin-3 in −Dox and +Dox treated 1012U cells. Dashed line depicts area of line-scan. Images taken from middle slice of Z-stack image. Scale bars = 10 μmm. (B) Profile plot of each signal in both conditions. Tracks normalized to a range of 100 to account for differences in nuclear size. N = 3, nuclei measured: −Dox 209, +Dox 179. (C) Percent of Emerin and Nesprin-3 (N3) signals within 3 μm of the nuclear periphery. Statistical significance calculated using Kruskal–Wallis test.