FIG. 4.
Induction of IFN-γ secreting T cells in NDV-treated tumor-bearing mice. Animals bearing CT26.CL25 tumors were treated as in Fig. 3. Tumor draining lymph nodes were pooled from 3 mice per group on day 14 post treatment initiation. (A) LN cells were dissociated and cocultured with irradiated CT26.wt cells or plated alone and assayed for IFN-γ production 3 days later (mean ± standard deviation, n=3). LN cells in media alone did not produce detectable IFN-γ (data not shown). (B) LN cells were cultured with 5ug/ml of minigal peptide or control peptide and assayed for IFN-γ production 3 days later. IFN-γ production from control peptide was undetectable (data not shown).