FIG. 3.
Color phenotypes of new [URE3] elements induced by overexpression. Clones isolated following overexpression of UPD are on the left, and clones isolated following overexpression of full-length Ure2p are on the right. Wild-type (wt) YMS23 and ure2 mutant (U11) cells are included as color controls. U46, U47, U57, and U58 are the clones used for phenotypic characterization (see text). Clones 1 to 8 and 9 to 16 were randomly selected from a second, independent overexpression experiment using the same plasmids. Colonies were picked from USA plates, purified, and streaked on YPD.