FIG. 7.
Identity of proteins involved in the formation of E2F complexes in DM and normal cells. (A) Gel shift/supershift analysis with nuclear extracts from predifferentiated cells. Antibodies to E2F4, p107, p130, and Rb (indicated at the top) were incorporated into the E2F reaction mixture with nuclear extracts from predifferentiated (day 0) control and DM cells. (B) Gel shift/supershift assay with nuclear extracts isolated on day 3 after the initiation of differentiation. Antibodies to E2F1 to E2F-4 and Rb family proteins were incorporated into the E2F binding reaction mixture.