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TGF-β1–induced actin reorganization: dependence on protein synthesis and comparison with the effect of HGF. PC-3U cells were starved in 1% FBS for 12 h and stimulated with 10 ng/ml TGF-β1 for 30 min or 48 h (A), 25 ng/ml HGF for 30 min or 48 h (B), or pretreated with 10 μg/ml cycloheximide for 1 h before TGF-β1 stimulation (C). Filamentous actin was visualized by TRITC-labeled phalloidin. Bar, 20 μm.
