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. 2023 Jan 23;3(1):100391. doi: 10.1016/j.crmeth.2022.100391

Figure 4.

Figure 4

Fungal recovery using ITS1 amplicon sequencing and shotgun metagenomics

(A) Boxplot shows the top 20 fungal species (based on total read recovery across all samples) determined using ITS1 amplicon sequencing and the DADA2 pipeline with the UNITE database.

(B) Venn diagram showing the concordance of the fungal species detected using the ITS1 data and the Rep200 pipelines (only this database detects fungus in the Qiita platform).

(C) Boxplot showing the read recovery for all samples across fungal species (x axis) as detected in the metagenomic sequences using FindFungi (log scale on the y axis).

(D) Boxplots for the relative abundance of the identified fungal species using Metaphlan2 (showing data from 15/1,772 samples had fungal reads identified, log scale on the y axis).