TABLE 4.
Hemolytic activity and confirmation of sly region genomic arrangements by PCRa
| Isolate or strain | Characteristic or test
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| sly | RFLP profile | HE activity | PCR with primer pairc
|
||||||
| 14 (orfB)-15 (orfD) | 3(sly)-11 (orfD) | 10(orfA)-2 (sly) | 12 (orfC)-11 (orfD) | 10 (orfA)-13 (orfC) | 12 (orfC)-13 (orfC) | ||||
| 47 | + | 1 | + | +d | + | + | − | − | − |
| 3 | + | 2 | + | − | + | − | − | − | − |
| 58 | + | 3 | + | +d | + | + | − | − | − |
| 35 | + | 4 | ±b | +d | + | + | − | − | − |
| 37 | + | 5 | + | +d | + | + | − | − | − |
| 73 | + | 6 | + | +d | + | + | − | − | − |
| 102 | + | 7 | − | +d | + | + | − | − | − |
| 122 | + | 8 | + | +d | + | + | − | − | − |
| 128 | + | 9 | + | − | + | + | − | − | − |
| 167 | + | 10 | − | +d | + | + | − | − | − |
| 230 | + | 11 | + | +d | + | + | − | − | − |
| 238 | + | 12 | + | − | + | + | − | − | − |
| 348 | + | 13 | + | +d | + | + | − | − | − |
| 352 | + | 14 | ±b | +d | + | + | − | − | − |
| 296 | + | 15 | − | +d | + | + | − | − | − |
| 242 | + | 16 | + | +d | + | + | − | − | − |
| 247 | + | 17 | + | − | + | + | − | − | − |
| 248 | + | 18 | + | +d | + | + | − | − | − |
| 275 | + | 19 | + | +d | + | + | − | − | − |
| 297 | + | 20 | + | +d | + | + | − | − | − |
| 99 | + | 21 | + | +d | + | + | − | − | − |
| P1/7 | + | + | +d | + | + | − | − | − | |
| DH5 | − | − | +e | − | − | + | + | + | |
| 36 | − | − | +e | − | − | + | + | + | |
| 44 | − | − | +e | − | − | + | + | + | |
| 91 | − | − | +e | − | − | + | + | + | |
| 109 | − | − | +e | − | − | + | + | + | |
| 119 | − | − | +e | − | − | + | + | + | |
| 141 | − | − | +e | − | − | + | + | + | |
| 151 | − | − | +e | − | − | + | + | + | |
| 160 | − | − | +e | − | − | + | + | + | |
| 346 | − | − | +e | − | − | + | + | + | |
a
Isolates shown represent 21 sly RFLP profiles, strain P1/7, and 10 sly-negative isolates (including DH5). HE, hemolytic; +, present; −, absent.
b
±, weakly hemolytic activity.
d
Large product (ca. 2,700 bp).
e
Small product (ca. 1,400 bp).