Fig. 6. Fecal SCFA and hepatic glycogen levels differed between exercised B. uniformis–monoassociated mice and germ-free mice.
(A) SCFA concentrations in the feces of exercised germ-free mice (n = 8) and B. uniformis–monoassociated mice (n = 7). (B) Hepatic glycogen content and expression of Cpt1a and Pck1 in exercised germ-free mice (n = 8) and B. uniformis–monoassociated mice (n = 7). The box-and-whisker plot illustrates the distribution of values within each group. An unpaired t test was used to statistically analyze the acetate, propionate, isovalerate, glycogen, and Cpt1a levels. The Mann-Whitney U test was used to analyze the valerate, isobutyrate, and Pck1 levels. These tests were selected on the basis of the outcomes of the normality test. All statistical tests were two-tailed.