. 2001 Aug;69(8):4891–4897. doi: 10.1128/IAI.69.8.4891-4897.2001

TABLE 2.

Oligonucleotide primers used in this study

Primer	Sequence (5′→3′)
UreA-F1	`ATGAAACTCACCCCAAAAGA`
UreA-R1-T7^a	`ctaatacgactcactatagggagaGGAAGTGTGAGCCGATTTGA`
UreI-F2	`AAGCACTGCGGTGATGAACT`
UreI-R2-T7^a	`ctaatacgactcactatagggagaACCAATCGCCTTCAGTGATG`
UreG-F1	`TGACATGGCGGTCATCACTA`
UreG-R-T7^a	`ctaatacgactcactatatagggagaCAAGTCTGAGCGCGTGATTC`
BJD3.3-F1^b	`cgcggatccTTTTTGAAGGGCATTTGTGC`
BJD3.3-R1^b	`cgcggatccAACTTGTCTAACTCTTTTGG`

Primers contained a 5′ extension with T7 promoter sequence (in lowercase letters) for the creation of an antisense RNA probe.

Primers contained a 5′ extension for cloning purposes, indicated in lowercase letters. A BamHI restriction site is underlined.