TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference(s) or source |
|---|---|---|
| C. burnetii | Nine Mile, phase I, RSA 493 | 42 |
| E. coli | ||
| DH5α | F− φ80d lacZΔM15Δ (lacZYA-argF) U169 deoR recA endA1 phoA relA1 | Gibco-BRL |
| BL21 | F−ompT hsdS (rB− mB−) gal, dcm plysE | Novagen |
| XL1-MRF′ | lac[F′ proAB lacIqZΔM15 Tn10] 2tetrΔ(mcrA)endA1 supE44 thi-1 recA1 gyrA96 relA1 | Stratagene |
| SOLR | F′ proAB lacIqZΔM15 Su− lac gyrA96 relA1 thi-1 endA1 λrsbcC recB recJ umuC::Tn5(kanr) uvrC | Stratagene |
| TOP10F′ | F′ [lacIq Tn10 (tetr)] lacZΔM15Δ deoR recA1 araD139 Δ(ara-leu)7679galU galK rpssL endA1 nupG mcrA | Invitrogen |
| MC4100 | F− Δ(argF-lac) U169 araD139 rpsL150 ptsF25 flbB5301 rpsR deoC relA1 | 46 |
| RO151 | MC4100 φ(csi-5::lacZ)(λplacMu55) | 49 |
| LM5003 | MC4100 rpoS359::Tn10 Tetr | 12, 17 |
| LM5004 | RO151, Kanr | 12, 49 |
| LM5005 | RO151 rpoS359::Tn10 Kanr Tetr | 12, 17 |
| Plasmids | ||
| pSKII(−) | Cloning vector, Apr | Stratagene |
| pCR2.1-TOPO | TA cloning vector, Apr | Invitrogen |
| pGEX-4T-1,2,3 | GST gene fusion vector, Apr | Amersham-Pharmacia Biotech |
| pSK 5-22 | pSK(−) with 1.8-kb EcoRI insert (rpoD) | This work |
| pR0S003 | pSK(−) with 4-kb HindIII insert (rpoS) | This work |
| pR0S104 | pCR2.1 with 953-bp L. pneumophila (partial) rpoS insert | This work |
| pR0S105 | pCR2.1 with 1,060-bp C. burnetii rpoS insert | This work |
| pR0S106 | pGEX 4T-1 with 1,060-bp rpoS insert in frame and correct orientation | This work |
| pR0S107 | pGEX 4T-2 with 1,060-bp rpoS insert | This work |
| pR0S108 | pGEX 4T-3 with 1,060-bp rpoS insert | This work |
| pLM507 | RSF1010 with 9.1-kb insert bearing L. pneumophila (complete) rpoS | 12 |