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. 2001 Aug;69(8):4874–4883. doi: 10.1128/IAI.69.8.4874-4883.2001

FIG. 1.

FIG. 1

Detection of C. burnetii rpoD by Southern blotting. The PCR-amplified 558-bp internal rpoD gene region from C. burnetii was used as a probe to hybridize with C. burnetii chromosomal DNA digests. (A) Probe hybridized with EcoRI digest (1.9-kb EcoRI fragment hybridized) (lane 1) and a 558-bp C. burnetii rpoD amplicon (lane 2). (B) L. pneumophila PCR-amplified rpoD was used as the probe and hybridized with C. burnetii EcoRI digest (1.9-kb EcoRI fragment hybridized) (lane 1) and a 2.0-kb L. pneumophila rpoD amplicon (lane 2).