TABLE 2.
Severity of neutrophil influx in mouse bladders 1 day after infection with CNF1-positive CP9 or its cnf1 isogenic mutant
| Straina | No. of CFU/gb | Avg no. of neutrophils (range)c | Severity of inflammationd |
|---|---|---|---|
| CP9 (6) | 104–107 | 95 (39–181) | 100 |
| CP9 cnf1 (5) | 105–107 | 39 (7–129) | 20 |
a
Numbers in parentheses are the numbers of mice tested.
b
Range of bacterial counts present in the bladders examined.
c
Neutrophils were counted within the tissue in the area of greatest inflammation, and PMN in blood vessels were excluded (magnification, ×600). Slides were coded and examined in a blinded fashion by a pathologist. All slides from CP9-infected animals were reported to contain many more areas of inflammation than the slides from animals infected with the CNF1-negative mutant. Inflammation was scored as described in Materials and Methods.
d
Percentage of animals with moderate to severe inflammation.