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Induction of macrophage gene expression by E. coli K235 LPS and P. gingivalis LPS in C3H/OuJ and C3H/HeJ macrophages. Total macrophage RNA was subjected to RT-PCR with Southern blotting for the detection of IL-1β, MIP-2, and HPRT mRNA as described in the text. HPRT served as the housekeeping gene in these experiments. The data are representative one of three separate experiments.
