TABLE 2.
Exponential-phase supernatant proteins identified by peptide mass fingerprinting
| Designation | Name | Accession no.a | Mr/pIb | No. of peptides (coverage)c | Δppmd |
|---|---|---|---|---|---|
| SpeB-25 | Heat shock protein | U72721 | 64,920/4.62 | 10 (18) | 2.9 ± 3.2 |
| Rgg-4 | Heat shock protein | X89236, RST0771 | 50,497/4.68 | 14 (37) | 4.0 ± 1.8 |
| Rgg-16 | DNA K | RST00473 | 58,144/4.58 | 6 (16) | 9.9 ± 5.4 |
| Rgg-29 | Enolase | RST00465 | 37,330/4.83 | 8 (34) | 12.2 ± 2.3 |
| Rgg-37 | Enolase | RST00465 | 37,330/4.83 | 7 (24) | 0.5 ± 2.3 |
a
NCBI accession number or WIT2 RST designation.
b
Inferred from the nucleotide sequence.
c
Number of tryptic peptides of the identified protein detected by MALDI-TOF MS (percentage of the protein covered with detected peptides).
d
Mean difference ± standard error between masses detected by MALDI-TOF MS and calculated masses of the tryptic peptides.