. 2001 Jan;69(1):221–227. doi: 10.1128/IAI.69.1.221-227.2001

TABLE 1.

Primer sequences used for PCR-assisted mRNA amplification

Target primer	Oligonucleotide sequence
IL-1α	5′`AAGATGTCCAACTTCACCTTCAAGGAGAGCCG`3′
	5′`AGGTCGGTCTCACTACCTGTGATGAGTTTTGG`3′
TNF-α	5′`TTCTGTCTACTGAACTTCGGGGTGATCGGTCC`3′
	5′`GTATGAGATAGCAAATCGGCTGACGGTGTGTGGG`3′
IL-6	5′`ATGAAGTTCCTCTCTGCAAGAGACT`3′
	5′`CACTAGGTTTGCCGAGTAGATCTC`3′
IFN-γ	5′`TGCATCTTGGCTTTGCAGCTCTTCCTCATGGC`3′
	5′`TGGACCTGTGGGTTGTTGACCTCAAACTTGGC`3′
IL-4	5′`ATGGGTCTCAACCCCCAGCTAGT`3′
	5′`GCTCTTTAGGCTTTCCAGGAAGTC`3′
IL-10	5′`CTGGGAAGACCAAGGTGTCTAC`3′
	5′`GAGCTGCTGCAGGAATGATGA`3′
MIP-2	5′`CTGCGCTGTCAATGCCTG`3′
	5′`AGCCTTGCCTTTGTTCAGTA`3′
β-actin	5′`GTGGGCCGCTCTAGGCACCAA`3′
	5′`CTCTTTGATGTCACGCACGATTTC`3′