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HPTLC (A) and HPTLC-immunostaining (B) of glycolipids obtained from serotype B (lanes a) and D (lanes b) cells or from cell wall preparations from cap 67 cells (lanes c) compared with the purified glucosylceramide from HEC3393 cells (lanes d). Orcinol-reactive bands with Rf values similar to that of the purified cryptococcal glucosylceramide were detected by HPTLC. Immunostaining showed that antibodies to CMH recognized components with migration similar to that of CMH.
