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Induction of GFP expression in vitro following growth under iron-limiting conditions. Wild type (A) or iron transport mutatnts (B) were subcultured 1:200 from an overnight culture into LSLB containing EDDA and grown to late exponential phase. Mean fluorescence values obtained from plasmids pGTXN3 and pSR2 were normalized to values obtained with the constitutive expression plasmid pLR9.
