Fig. 4. Increased food intake and obesity in mice genetically deficient in Lac-Phe.
(a) Plasma Lac-Phe levels in male WT (blue) and CNDP2 KO (red) mice under sedentary conditions or after a single bout of exhaustive treadmill running.
(b,c) Cumulative daily food intake (b) and body weight (c) of male WT (blue) and CNDP2-KO (red) mice under an obesigenic diet/exercise training regimen in which mice were fed high fat diet (60% kcal from fat) and exercised by treadmill running 5 days/week (see Methods).
(d) Change in body weight of WT (blue) and CNDP2-KO (“KO”, red) mice after 40 days of obesogenic diet alone (“HFD/Sedentary”) or obesogenic diet with treadmill running (“HFD/Exercise”).
(e,f) Tissue weights (e) and representative images of adipose tissues (f) of WT (blue) and CNDP2-KO (red) mice following a combined obesogenic diet/treadmill running protocol. Tissue weights and images were taken on day 41. iWAT, inguinal white adipose tissue; eWAT, epididymal white adipose tissue; BAT, brown adipose tissue.
For (a), N=6/group. For (b,c) and (e,f), N=8 for WT and N=8 for CNDP2-KO. For (d), N=8 for WT HFD/Sedentary, N=11 for CNDP2-KO HFD/Sedentary, N=9 for WT HFD/Exercise, and N=8 for CNDP2-KO HFD/Exercise. Data are shown as mean ± SEM. The experiments shown in (a-c) were performed two times and the experiments shown in (d-f) were performed once. P-values were calculated by two-way ANOVA with repeated measures (for b,c) or Student’s two-sided t-test (for a, d, and e).