Extended Data Fig. 8. Additional characterization of plasma Lac-Phe levels in humans.
(a,b) Tandem MS fragmentation (a) and co-elution (b) of an authentic Lac-Phe standard and the endogenous m/z = 236.0928 mass from human plasma run on the Snyder laboratory untargeted metabolomics platform (see Methods).
(c) Time course of phenylalanine levels in blood before and after a single acute bout of treadmill running from the human acute treadmill exercise study (Cohort 1, N=36).
(d) Time course of lactate levels before and after sprint (red), resistance (blue), and endurance (light blue) exercise from the human crossover acute exercise study (Cohort 2, N=8).
For (c,d), data are shown as mean ± SEM, **p < 0.01, ***p < 0.001.
Experiments were performed once. P-values were calculated by two-way ANOVA with repeated measures.