Figure 2.
Schematic protocol for the isolation and identification of cardiac fibroblasts: 11–12 week-old C57BL/6J and BALB/c female wild-type (WT) mice were sacrificed, and the following process was executed separately on each mouse: the heart was extracted and washed with PBS. Aorta and atriums were removed, and the ventricles were then minced and enzymatically digested using HBSS complemented with a mixture of collagenase I, II, and DNAse, followed by 70 µm and 40 µm filtrations and FC blocking. Live/dead staining and staining with the antibody MEFSK4 that is specific for CFs enabled us to examine them using flow cytometry. Figure was created using BioRender.com.