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. 2022 Dec 16;6(1):e202201701. doi: 10.26508/lsa.202201701

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© 2022 Liu et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — (A) Low-resolution image of the DAPI channel for a mouse kidney tissue sample. Red box indicates zoomed-in region displayed in B-E. (B, C) DAPI (B) and (C) cell boundary antibody stain channels for the zoomed-in region. (D) Multiplexed error-robust fluorescence in situ hybridization signal for a single barcode bit channel in the same zoomed-in region. (E) Positions of decoded mRNA transcripts (colorful dots) and segmented cell boundaries (black) in the same zoomed-in region after running data through image analysis pipeline, with each color representing a unique gene species. Panels (B, C, D) show raw images with brightness and contrast levels selected for ease of visualization.