FIG. 5.
Consensus sequences for choline-binding regions of PspC and PspA. The repeat regions of eight proteins, which included three PspA and five PspC molecules, were aligned with the Clustal W algorithm. The alignment was adjusted to maintain divergent repeats together to account for the various sizes of the choline-binding domains. The consensus for repeat number 1 through repeat number 9 of the PspA proteins is in the upper half of figure and that of the PspC proteins is in the bottom half of the figure. The consensus for all nine repeats (1–9) representing at least 60% of the individual amino acids in each position is shown at the top of the diagram. The amino acids that diverge from the consensus sequence are indicated in light gray, and the C-terminal tail is indicated in dark gray. Five amino acids that vary in a gene-specific manner are indicated in white letters on a black background.