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. 1999 Nov;67(11):6026–6033. doi: 10.1128/iai.67.11.6026-6033.1999

TABLE 1.

Strain or plasmid Relevent characteristics Source or reference
Strains
 C. burnetii Nine Mile, phase I, RSA 493 23
 C. trachomatis Biotype E American Type Culture Collection
 E. coli DH5α F− φ80d lacZΔM15Δ(lacZYA-argF)U169 deoR recA endA1 phoA supE44 λ−thi-1 gyrA96 relA1 GIBCO/BRL
 E. coli JM109 F′ traD 36 proAB lacIqZ Δ M15 recA1 endA1 gyrA96 thi-1 relA1 supE44 Δ(lac-proAB) Promega
 E. coli XL1-MRF′ lac (F′ proAB lacIqZΔM15 Tn10) (Tetr ΔmcrA) 183Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 Stratagene
 E. coli SOLR F′ proAB lacIqZΔM15 Su−(nonsupressing)lac gyrA96 relA1 thi-1 endA1 λRsbcC recB recJ umuC::Tn5(Kanr) uvrC Δ(mcrCB-hsdSMR-mmr)171 Stratagene
 E. coli TOP10F′ F′[lacIq Tn10 (Tetr)] lacZΔM15 ΔdeoR recA1 araD 139 Δ(ara-leu)7679 galU galK rpsL endA1 nupG mcrA Δ(mrr-hsdRMS-mcrBC)φ80 Stratagene
Plasmids
 pSKII(−) Cloning vector Apr Stratagene
 pB1-7 pSKII(−) with 9-kb rpsB-tsf insert This work
 pB1-7-L pSKII(−) with 4-kb EcoRI insert from pB1-7 This work
 pB1-7-B pSKII(−) with 5-kb EcoRI insert from pB1-7 This work
 pGEM-T PCR cloning vector Apr Promega
 pROS100 pGEM-T with 900-bp tuf insert This work
 pGEX-4T-1 GST gene fusion vector Apr Pharmacia
 pROS101 pGEX4T-1 with 900-bp tuf insert This work
 pROS102 pGEX4t-1 with 900-bp tuf insert (opposite orientation) This work
 pCR2.1 TA cloning vector Apr Invitrogen
 pROS103 pCR2.1 with 1,100-bp tuf insert This work