FIG. 6.
Expression of GST fusion protein. Western blot of partial tuf clones in pGEX4T-1 was probed with anti-GST antibody to confirm expression of recombinant EF-Tu. Lane 1, purified GST; lane 2, uninduced pROS102 (gene inserted in nonexpressing orientation); lane 3, IPTG-induced clone pROS102 did not express a GST-fusion protein; lane 4, IPTG-induced clone pROS101 (gene inserted in correct orientation) expressed a ∼65-kDa GST-EF-Tu fusion protein; lane 5, uninduced clone pROS101 did not express a significant amount of the fusion protein; lane 6, identically prepared sample as in lane 4.