FIG. 2.
PAGE analysis of A. hydrophila exoproteins. (A) Denaturing PAGE of TCA-precipitated proteins present in the supernatant of an overnight culture. C4-HSL-dependent proteins are arrowed. (B) Nondenaturing PAGE of Microcon 10-concentrated supernatant proteins. Proteins predicted to be multimerized aerolysin and serine protease are arrowed. (C) Casein zymogram of supernatant proteins. Activity predicted to be due to the serine protease is arrowed. (D) Gelatin zymogram of supernatant proteins. Activity predicted to be due to the serine protease is arrowed. In each case, lane 1 is a sample derived from A. hydrophila AH-1N, lane 2 is from A. hydrophila AH-1NahyI-6, lane 3 is from A. hydrophila AH-1NahyI-6 cultured with 5 μM C4-HSL, and lane 4 is from A. hydrophila AH-1N cultured with 10 μM 3-oxo-C12-HSL. M denotes standards, with molecular masses in kilodaltons indicated.