FIG. 6.
Detection of B. burgdorferi DNA in tissues from infected mice. Mice were infected by intradermal injection of 2,000 B. burgdorferi organisms, and the indicated tissues were harvested 4 weeks postinfection. Tissues were assessed for B. burgdorferi-specific DNA by continuously monitored PCR of the B. burgdorferi recA gene, and results were normalized to those of the host nidogen gene. Each data point represents the B. burgdorferi-specific DNA content of an individual infected tissue relative to nidogen, and each black bar indicates the average score for the eight mice in a group. Uninfected controls contained no B. burgdorferi-specific DNA (data not shown). These results are representative of two separate experiments.