FIG. 2.
RT-PCR products of mRNA from biopsied (A) and autopsied (B) tissues, as demonstrated by gel electrophoresis. The 200-bp fragments represent the presence of mRNA for HBD-1, and the 300-bp fragments represent the control APRT mRNA product. (A) Lanes: 1, negative control (running the RT procedure with sterile water); 2, RT-PCR of mRNA collected from an EAC skin sample; 3, control tissue sample from skin; 4, PCR product obtained by amplification of the cDNA for HBD-1 inserted in the pCR3.1 plasmid. (B) Lanes: 1, RT-PCR products from an autopsied tissue fragment of EAC skin; 2, pars tensa of the TM; 3, pars flaccida of the TM; 4, negative control; 5, amplification product of the HBD-1 cDNA.