FIG. 2.
Light microscopy and TEM of T. cruzi-infected human DC. (A) DC were incubated with T. cruzi trypomastigotes (parasite-to-cell ratio, 20:1) for 24 h, fixed with methanol, and stained with Giemsa stain. T, trypomastigote; arrows, parasite inside a parasitophorous vacuole; arrowheads, parasite in the cytosol. Bar, 2.5 μm. (B) DC were incubated with T. cruzi trypomastigotes (parasite-to-cell ratio, 5:1) for 24 h and treated for TEM. T, trypomastigote; arrow, parasite in the cytosol; N, DC nucleus. Bar, 1.5 μm.