TABLE 3.
Effect of kinase inhibitors on H. pylori-induced IL-8 transcription and IL-8 protein secretion in L5F11 gastric epithelial cells
| Inhibitor | Inhibitor concn (μM) | Luciferase activity (cpm, 106) (n = 5)a | IL-8 secretion (ng/ml) (n = 3–5)a |
|---|---|---|---|
| None (H. pylori NCTC 11637) | 1.51 ± 0.23 | 3.22 ± 0.59 | |
| Herbimycin A | 1 | 0.71 ± 0.18* | 0.83 ± 0.41* |
| H7 | 50 | 1.05 ± 0.42 | 0.91 ± 0.3** |
| Calphostin C | 1 | 1.63 ± 0.33 | 2.55 ± 0.24 |
| KT5823 | 100 | 1.62 ± 0.33 | 2.9 ± 0.48 |
a
Both luciferase activity and IL-8 protein concentrations are expressed as means ± SEMs after subtraction of the respective background cell controls. *, P < 0.05; **, P < 0.01 (compared with H. pylori stimulation in the absence of kinase inhibitors).