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. 2022 Sep 21;11:e77643. doi: 10.7554/eLife.77643

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© 2022, Boehm et al

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Figure 5. — (A) In vivo whole brain lightfield calcium imaging upon stimulation with putrescine or vinegar (nsyb-Gal4; UAS-GCaMP6m). Putrescine and vinegar activate different areas in the lateral horn (LH, dotted line). (B) Electron microscopy (EM)-based reconstruction showing projection neurons (PN) innervating the VC5 glomerulus. (C) VC5 PNad (pink) innervates the LH and the mushroom body (MB). (D) T-maze behavioral screen at restrictive temperature at test (30 °C) of candidate LHONs in mated females reveals that two LHON types are required for full attraction to putrescine. ‘empty x shi’ represents the control group and stands for pBDPGAL4;UAS-shi^ts1, the Gal4 vector used to generate all MB-Gal4 lines without an expression driving enhancer. One-way Anova, Bonferroni corrected; p-values: *:<0.05.

Figure 5—figure supplement 1. — (A) In vivo whole brain lightfield calcium imaging upon stimulation with putrescine or vinegar (nsyb-Gal4; UAS-GCaMP6m). Putrescine and vinegar activate different areas in the lateral horn (LH, dotted line). (B) Electron microscopy (EM)-based reconstruction showing projection neurons (PN) innervating the VC5 glomerulus. (C) VC5 PNad (pink) innervates the LH and the mushroom body (MB). (D) T-maze behavioral screen at restrictive temperature at test (30 °C) of candidate LHONs in mated females reveals that two LHON types are required for full attraction to putrescine. ‘empty x shi’ represents the control group and stands for pBDPGAL4;UAS-shi^ts1, the Gal4 vector used to generate all MB-Gal4 lines without an expression driving enhancer. One-way Anova, Bonferroni corrected; p-values: *:<0.05.