TABLE 1.
Summary of phenotypic characterization of a CSH1 knockout strain
| Strain | Doubling time (min) (mean ± SD)a | 50% culture germination (min) (mean ± SD)b | CSH (mean ± SD)c
|
|
|---|---|---|---|---|
| 23°C | 37°C | |||
| CAI4 | 68 ± 0.2 | 90 ± 2.1 | 96.6 ± 2.9 | 52.8 ± 20.3 |
| csh1/csh1 | 79 ± 0.0 | 86 ± 5.7 | 26.3 ± 13.2 | 7.3 ± 4.6 |
a
Doubling time represents estimates (in minutes) of the generation time of duplicate exponentially growing liquid cultures.
b
Percent culture germination was observed at timed intervals, and the time where 50% of the cells in a given sample microscopically demonstrated pseudohyphal or germ tube morphology was extrapolated from the time course.
c
Relative CSH of individual cells from cultures at a given temperature is presented as the percentage of cells in a sample showing the hydrophobic phenotype. CSH measurements represent samples counted in triplicate in each experiment, and each experiment was repeated at least six times.