FIG. 1.
Putative epitope of MAb 6C5-H4CA antigen. (A) The conceptual translation of the DNA sequence encoding random peptides was aligned using the GCG module PILEUP, and the putative epitope of the antigen was determined. (B) Immunoreactivity of soluble GST fusion proteins was tested on glutathione-agarose-purified material by Western blotting. Lane A, an arbitrarily chosen clone from the fifth round of panning that did not react with MAb 6C5-H4CA; lanes B, C, and D, immunopositive clones 1–8, 4–3-3, and 5–4-1 from panel A, respectively.