TABLE 1.
Bacterial strains and plasmids
| B. subtilis strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| JH642 | pheA1 trpC2 | J. A. Hoch |
| EU8701 | pheA1 trpC2 ΔsigE::Ermr | C. P. Moran |
| MH5636 | pheA1, trpC2, and rpoCΩpYQ52, Cmr | 20 |
| MH5654 | pheA1, trpC2, and rpoCΩpYQ52, Cmr ΔsigE::Ermr | Y. Qi |
| Plasmids | ||
| pCR2.1 | Vector for cloning PCR products (Ampr Kanr) | Invitrogen |
| pXH24 | Ampr Kanr (4.0 kb), ctaA promoters A2 and A3 (180 bp) in pCR2.1 | 29 |
| pXH37 | Ampr Kanr (4.0 kb), ctaA promoter A2 (163 bp) in pCR2.1 | 29 |
| pXH38 | Ampr Kanr (3.9 kb), ctaA promoter A3 (122 bp) in pCR2.1 | 29 |
| pXH43 | Ampr Kanr (4.1 kb), ctaA promoters A2 and A3 (224 bp) in pCR2.1 | 29 |