Figure 4: PhyloCSF analysis of the mouse 1810058I24Rik gene, which encodes the microprotein mitolamban/Stmp1/Mm47.
(A) The mouse 1810058I24Rik gene is comprised of three exons, and the arrowheads in the intronic regions point right, indicating it is transcribed on the plus strand of DNA and therefore the PhyloCSF +1, +2, and +3 tracks should be analyzed. The conserved microprotein coding sequence spans all three exons, starting in exon 1 (B), reading through exon 2 (C), and ending in exon 3 (D). Note that the positive PhyloCSF score is found on the +2 track in exon 1, the +3 track in exon 2, and the +2 track in exon 1. The reason for the movement of the positive score from one track to the other is that PhyloCSF analyzes the six potential reading frames of the DNA sequence independent of the gene’s exon/intron structure. Therefore, an intron containing a number of nucleotides that is not divisible by three (three nucleotides/codon) will cause a shift in the reading frame to a different track.