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Analysis of the mutagenized fur gene by Southern hybridization. HindIII- and Sau3A-digested DNAs from the strain NCTC11638 (lanes 1) and the fur mutant NCTC11638-FUR (lanes 2) were hybridized with probes specific for fur and cat, respectively. The arrows indicate hybridizing DNA fragments of the predicted sizes, demonstrating correct replacement of the wild-type fur gene with the interrupted version. The sizes of individual marker DNA fragments are indicated on the left.
