TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Acinetobacter | ||
| ADP1 | Wild type | 17, 34 |
| WH362 | rubA(ΔMscI-Tth111I)::lacZ Kmr | 9 |
| WH363 | EMS mutant, alk | 9 |
| WH380 | ORFX(MscI)::lacZ Kmr | This study |
| WH382 | rubB(ΔNarI-NruI)::lacZ Kmr | This study |
| WH384 | estB(ΔNsiI-StyI)::lacZ Kmr | This study |
| WH384ΔoxyR | estB(ΔNsiI-StyI)::lacZ oxyR(ΔAflII-NdeI) Kmr Cmr | This study |
| WH386 | oxyR(ΔAflII-NdeI)::lacZ Kmr | This study |
| WH432 | EMS mutant, alk | 9 |
| WH433 | EMS mutant, alk | 9 |
| WH434 | EMS mutant, alk | 9 |
| E. coli DH5α | recA1 endA1 supE44 gyrA96 thi hsdR17(rK−mK−) relA1 φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 | 13 |
| Plasmids | ||
| pAKA22 | Apr Tcr; 11.6-kbp Sau3A fragment of Acinetobacter DNA containing estB in BclI site of pUN121 | 19 and this study |
| pBluescript II SK+ | Apr | Stratagene, La Jolla, Calif. |
| pKOK6.1 | Apr Kmr; promoterless lacZ | 21 |
| pKT210 | Cmr | 5 |
| pWH891 | Apr; 10,855-bp Acinetobacter DNA | 10 |
| pWH891ΔAflII | Apr; pWH891, deletion of AflII fragment (4,348 bp) containing ORFX, rubA, rubB, and estB | This study |
| pWH891ΔNcoI | AproxyR | This study |
| pWH891SK3 | Apr; HindIII-PvuII fragment (3,079 bp) from pWH891 in EcoRV site of pBluescript II SK+ | This study |
| pWH891SK3ORFX::lacZ | Apr Kmr; ORFX(MscI)::lacZ fusion | This study |
| pWH891SK6 and SK6i | Apr; NruI-EcoRV fragment (2,149 bp) from pWH891 in EcoRV site of pBluescript II SK+ | This study |
| pWH891SK6estB::lacZ | Apr Kmr; estB(ΔNsiI-StyI)::lacZ fusion | This study |
| pWH891SK7i | Apr; HindIII-BamHI fragment (4,727 bp) from pWH891 in EcoRV site of pBluescript II SK+ | This study |
| pWH891SK7ioxyR::Cmr | Apr Cmr; oxyR(ΔAflII-NdeI)::Cmr fusion | This study |
| pWH891SK7ioxyR::lacZ | Apr Kmr; oxyR(ΔAflII-NdeI)::lacZ fusion | This study |
| pWH963ΔMH | Apr; MscI-DraI fragment (1,382 bp) from pWH891 in EcoRV site of pBluescript II SK+ | This study |
| pWH963ΔMHrubB::lacZ | Apr Kmr; rubB(ΔNarI-NruI)::lacZ fusion | This study |