FIGURE 5.
Pair-wise aggregation kinetics comparison of 3R and 4R tau isoforms. (A, B) Quantitative analysis of tau isoform aggregation kinetics using ThT fluorescence-based assays. 30 μM of 0N3R or 0N4R (Left Panel), 1N3R or 1N4R (Center Panel), and 2N3R or 2N4R (Right Panel) were incubated in 20 mM Tris pH 7.4 with 0.06 mg/ml of heparin at 37°C for specified time periods. Kinetic fluorescence traces are shown in panel (A). Corresponding t1/2 values for each aggregation kinetics curves are shown in panel (B). (C) Aggregation kinetics comparison of 0N3R and 0N4R isoforms by Sarkosyl-insoluble tau pelleting assay. 30 μM of 0N3R or 0N4R were incubated in 20 mM Tris pH 7.4 with 0.06 mg/ml of heparin at 37°C for specified time periods. Aggregated tau isoforms (Sarkosyl-insoluble tau) were visualized by SDS-PAGE after Commassie Blue staining.