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. 2001 Jul;67(7):3122–3126. doi: 10.1128/AEM.67.7.3122-3126.2001

TABLE 3.

Mean CT values and standard deviations of a dilution series of the nuc gene of S. aureus using the TaqMan probe with the LightCycler or the ABI PRISM 7700 SDS and different reaction mixes

System Mean CT value ± SD
3 × 100 copies/μl 3 × 101 copies/μl 3 × 102 copies/μl 3 × 103 copies/μl 3 × 104 copies/μl
LightCycler with a commercial kit 36.72 ± 0.128 33.02 ± 0.240 29.80 ± 0.105 26.46 ± 0.167 22.32 ± 0.147
LightCycler with Platinum Taq DNA polymerase 34.65 ± 0.214 31.11 ± 0.243 27.99 ± 0.064 24.76 ± 0.068 21.29 ± 0.052
ABI PRISM 7700 SDS with Platinum Taq DNA polymerase 35.07 ± 0.367 31.49 ± 0.069 28.56 ± 0.208 25.74 ± 0.121 NMa
a

NM, not measured.