Figure 7. f-Chrimson DNA was not detected in inner organs of mice.
(A) pAAV vector used in the study. Arrows indicate forward (F) and reverse (R) primers. (B) Agarose gel image of PCR products amplified from template plasmid DNA (pAAV-hSyn-f-Chrimson-eYFP-WPRE-bGH) as positive control for amplified site (1), double-distilled H2O as PCR negative control (2), tissue DNA sample extracted from contralateral non-injected cochlea as DNA isolation control (3), tissue DNA samples (dashed rectangle) extracted from contralateral non-injected cochlea (4), injected cochlea (5), heart (6), lung (7), liver (8), kidney (9), and spleen (10) are shown. All tissue samples are from a 1-mo-old transduced mouse. Expected PCR product size for cochleae and the inner organs is 917 bp (4–10) whereas it is 367 bp for DNA isolation control (3).