Figure 3.
LDHA mediates METTL3-regulated metabolic reprogramming in 5-FU resistant cells. (A) The mRNA expression profiles of critical enzymes for glycolysis in sh-NC or sh-METTL3 CRC/5-FU cells. (B & C) The mRNA (B) and protein (C) expression of LDHA in 5-FU resistant CRC cells treated with STM2457 (1 µM) for 24 h. (D) HCT-116 cells were transfected with pcDNA vector, pcDNA/METTL3, or pcDNA/METTL3-Mut for 24 h, the levels of LDHA and METTL3 were checked. (E-H) sh-NC or sh-METTL3 HCT-116/5-FU cells were transfected with pcDNA vector or pcDNA/LDHA for 24 h, the expression of LDHA was checked (E), and then the ATP generation (F), consumption of glucose (G), and production of lactate production (H) were measured. (I-L) sh-NC or sh-METTL3 SW480/5-FU cells were transfected with pcDNA vector or pcDNA/LDHA for 24 h, the expression of LDHA was checked (I), and then the ATP generation (J), consumption of glucose (K), and production of lactate production (L) were measured. Data are presented as means ± SD of three independent experiments. ** p < 0.01 compared with control; NS, no significant.