Table 2.
Antibodies used in this study.
| Name | Description | Dilution | Company | Catalogue# |
|---|---|---|---|---|
| Primary antibody | ||||
| Anti‐Sox2 | Rabbit polyclonal | 100x | Abcam | ab97959 |
| Anti‐Tfap2 (6E4/4) | Mouse monoclonal | 300x | Santa Cruz | SC12762 |
| Anti‐H3K27me3 | Mouse monoclonal | 500x | Active Motif | 61017 |
| Anti‐Sycp3 | Mouse monoclonal | 100x | Abcam | ab97672 |
| Anti‐γH2A.X S139 | Rabbit polyclonal | 100x | Abcam | ab11174 |
| Anti‐Dazl | Rabbit polyclonal | 200x | Abcam | ab34139 |
| Anti‐GFP | Chicken polyclonal | 500x | Abcam | ab13970 |
| Surface markers | ||||
| SSEA1‐eFluor 660 | Mouse monoclonal | 50x | Thermos | 50‐8813‐42 |
| CD61‐PE‐Vio770 | Hamster monoclonal | 10x | Miltenyi Biotec | 130‐102‐627 |
| Secondary antibody | ||||
| Anti‐chicken IgY | Goat polyclonal / Alexa488 | 500x | Life Technologies | A11039 |
| Anti‐rabbit IgG | Goat polyclonal / Alexa488 | 500x | Life Technologies | A11034 |
| Anti‐mouse IgG | Goat polyclonal / Alexa555 | 500x | Life Technologies | A21424 |
| Anti‐rabbit IgG | Donkey polyclonal/Alexa647 | 500x | Life Technologies | A31573 |